Objective To investigate the expression of family with sequence similarity 96 member A(FAM96A)in the liver cancer tissues and to evaluate its effects on the cell proliferation and apoptosis of human liver cancer cells HepG2. Methods Real-time PCR and Western blotting were applied to detect the mRNA and protein expression levels of FAM96A in the liver cancer tissues and their adjacent tissues,HepG2 cells and L02 cells. Furthermore,the recombinant plasmid of pcDNA3. 1-myc-FAM96A containing the whole sequence of human FAM96A gene (FAM96A group)and pcDNA3. 1-vector pcDNA3. 1-vector blank plasmid (control group)were respectively transfected into HepG2 cells. Then MTT essay and flow cytometry were applied to detect the proliferation and apoptosis rate of HepG2 cells in the two groups. Results FAM96A mRNA levels were significantly decreased in the liver tissues than in the adjacent tissues (0. 704 ± 0. 178 vs. 1. 707 ± 0. 267)and in HepG2 cells than in L02 cells (0. 627 ± 0. 251 vs. 1. 654 ± 0. 285),(both P < 0. 05). While FAM96A protein expression levels were lower in the liver tissues as compared with those of the adjacent tissues and were lower in HepG2 cells than in L02 cells (both P < 0. 05). Moreover,MTT assay showed that the proliferation of the FAM96A group was significantly lower than that of the control group (P < 0. 05). FACS analysis indicated that the apoptotic rate was high-er in the FAM96A group than in the control group (33. 06% ± 3. 15% vs. 5. 08% ± 0. 75%),(P < 0. 05). Conclusion The fusion protein SMP30-IP10 eukaryotic expression plasmid is successfully con-structed,and it can inhibit the migration and invasion abilitiesof hepatoma cells,but has no effect on the cell proliferation.%目的 观察96序列相似的家庭成员A(FAM96A)在人肝癌组织中的表达并探讨其对人肝癌细胞系HepG2细胞增殖及凋亡的影响.方法 采用实时荧光定量聚合酶链反应及免疫印迹法检测FAM96A蛋白在肝癌组织和癌旁组织、HepG2细胞和L02细胞中的mRNA及蛋白表达量.采用脂质体转染法将含人FAM96A全长序列的pcDNA3.1-myc-FAM96A重组质粒转染至HepG2细胞中,取对数生长期的HepG2细胞,24 h后分为FAM96A组和对照组,分别转染pcDNA3.1-myc-FAM96A质粒和pcDNA3.1-vector空白质粒.采用MTT法及流式细胞技术检测两组HepG2细胞增殖活力和凋亡率.结果 FAM96A mRNA在肝癌组织、癌旁组织中的相对表达量分别为0.704±0.178、1.707±0.267,在HepG2细胞、L02细胞中的相对表达量分别为0.627±0.251、1.654±0.285,两者比较,P均<0.05.FAM96A蛋白在肝癌组织中的表达低于癌旁组织、在HepG2细胞中的表达低于L02细胞,P均<0.05.FAM96A组细胞增殖活力低于对照组(P<0.05),FAM96A组、对照组细胞凋亡率分别为33.06%±3.15%、5.08%±0.75%,两组比较,P<0.05.结论 FAM96A在人肝癌组织中表达下降,过表达FAM96A可抑制肝癌细胞增殖且诱导其凋亡.
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