一个猪场高致病性 PRRSV 的分离鉴定

摘要

本研究对一个猪场高致病性 PRRSV 疑似病例进行了病理剖检、PRRSV N 蛋白基因 RT －PCR 扩增以及序列测定和分析。结果表明，N2号分离株 N 蛋白核苷酸序列与高致病性毒株（JXA1株、HuN4株、SX －1株、TJM株）的一致性均为99．1％，N3／N4号与高致病性毒株的一致性均为98．9％，由此判定该猪场病猪感染毒株为高致病性 PRRSV。该研究结果可为养猪生产中高致病性 PRRSV 感染的判断提供借鉴。%The suspected cases of highly pathogenic PRRSV from a pig farm were conducted autopsy,the gene sequence of N -protein of PRRSV was amplified by RT -PCR,and then its sequence was determined and analyzed.The identity of the nucleotide sequence of N -protein was 99.1% between the isolate N2 and the highly pathogenic strains (JXA1,HuN4,SX -1 and TJM),and that of isolate N3 /N4 was 98.9% with the highly pathogenic strains.The results showed that the infected virus was highly pathogenic PRRSV in the pig farm.This research provided a reference for the diagnosis of highly pathogenic PRRSV infection in swine production.