花生突变体的诱导与筛选利用

摘要

Germplasm resources innovation through mutagenesis is an important way for crop genetic im-provement and gene identification.In this study, peanut mutant population was generated by treating mature seeds of ten peanut cultivars, such as Fenghua 1, Shanhua 13 and Weihua 10, with EMS, 60 Co γradiation and neutron irradiation.And totally 30 000 M1 lines were obtained.The phenotypes of M2 mutants were ana-lyzed and some that apparently changed in leaf, plant shape, pod and seed were identified.Those M2 mutants with obvious variations in phenotype were grown to generate M3 for further confirmation.These mutants were excellent materials for peanut genetic improvement and functional genomics studies in the future.%利用诱变创新种质材料是作物遗传改良和功能基因组学研究的重要途径。本研究以丰花1号、山花13号、潍花10号等10个主要推广的栽培花生品种为材料，利用EMS、60 Coγ射线、快中子辐射三种方法对成熟种子进行诱变处理，获得了一个含有30000多个M1代单株的诱变群体。对M2代突变体进行田间观察和近红外分析，初步筛选得到多份在叶、株型、荚果、种子发育等方面发生明显变异的材料。将具有明显性状变异的M2代突变体进行种植繁育获得M3代，进一步鉴定其表型变异的稳定性。这些材料的获得为花生遗传改良和功能基因鉴定提供了丰富的试验材料。