5-氟尿嘧啶对人绒毛膜癌细胞株JA R作用的观察

摘要

目的：观察5‐氟尿嘧啶（5‐Fu）对人绒癌细胞株JAR的体外作用。方法：采用M T T比色分析法，观察20、50、100μg／L 3种剂量的5‐Fu对人绒癌细胞株JA R细胞的生长抑制情况；通过免疫组化法检测3种浓度5‐Fu作用于JA R细胞后增殖细胞核抗原Ki67的表达情况。结果：①5‐Fu能抑制JAR细胞的生长，并在一定时间及浓度范围内，表现出剂量依赖性，72h内5‐Fu浓度在25～100μg／L时抑制作用比较明显；②100μg／L的5‐Fu作用于JA R细胞72h后细胞抑制率达73．19％，作用后增殖细胞核抗原Ki67明显下降。结论：①5‐Fu对人绒癌细胞株JA R细胞的生长具有抑制作用，72h内呈剂量依赖性；②5‐Fu抑制了人绒癌细胞株JAR细胞的增殖，细胞可能被阻滞于G0及G1期。%Objective:To investigate the effects of 5‐FU on cell of human choriocarcinoma cell line JAR . Methods :The inhibitory effects of three different concentration of chemotherapeutical drug 5‐Fu(5‐Fluorouracil) (20 ,50 ,100μg/L) on JAR strain were assessed with methylthiazolyl tetrazolium MTT colorimetric assay .The ex‐pression of nuclear proliferative antigen Ki67 in JAR cells were detected with avidin‐biotin complex method ,immu‐no‐histochemical method .Results :① 5‐Fu inhibited the JAR cell growth in a dose‐dependent manner .This effect was obvious at 25~100μg/L in 72h .② When the JAR cells were exposed to 100μg/L 5‐Fu for 72h ,the expression of Ki67 antigen was declined significantly ,the inhibition rate was 73 .19% .Conclusion:① 5‐Fu inhibited the JAR cell growth in a dose‐dependent manner at 72h .② 5‐Fu inhibited the Proliferation of JAR cell ,which may be inhibi‐ted at the Period of G0 and G1 .