栀子苷与薯蓣皂苷对脑缺血再灌注大鼠急性期和恢复早期炎性损伤机制研究

摘要

Objective:Exploration of Geniposide and Dioscin on cerebral ischemia reperfusion injury in rats after acute stage and recovery effect of early inflammatory injury.Methods:The rats were divided into 4 groups such as:sham group, model group , drug group and Xingnaojing 10ml/kg group.Copy ischemia-reperfusion model after administration of each drug, evaluate the score of nerve injury, determine COX-2 and 5-LOX activity in brain tissue.Results:Dioscin and Geniposide can improve the scores of nerve damage, reduce activity of COX-2 and 5-LOX in brain homogenates.Conclusion:Dioscin and Geniposide have protective effect on cerebral ischemia-reperfusion injury in the acute phase through regulating the activity of COX-2 and 5-LOX protein in the four arachidonic acid pathway.%目的:探索栀子苷与薯蓣皂苷对脑缺血大鼠再灌注损伤后的急性期和恢复早期炎性损伤的影响.方法:将大鼠分为假手术组、模型组(再灌注24 h和72 h)、给药组(栀子苷和薯蓣皂苷分别设置30 mg/kg、60 mg/kg组)和阳性药组(醒脑静10 ml/kg组 )并采用线栓法灌胃给药后造模,对脑缺血再灌注后的急性期(24 h)和恢复早期(72 h)大鼠整体行为学评估、脑组织海马区的形态学观察以及脑组织匀浆中炎症介质COX-2和5-LOX蛋白活力检测等指标进行检测观察.结果:栀子苷与薯蓣皂苷能显著提高神经损伤评分,降低脑内COX-2和5-LOX的活力.结论:栀子苷与薯蓣皂苷通过对花生四烯酸通路中COX-2和5-LOX蛋白活力的调节对大鼠脑缺血再灌注后急性期(24 h)和恢复早期(72h)的炎性损伤具有一定的治疗作用.