1～100 kDalton的胶原水解物的分子量分布测定的研究

摘要

An attempt was made to use CB - peptides as new makers and to optimise the gel formulas and electrophoresis conditions for SDS -polyaerylamide gel electrophoresis to char~erise the acid -hydrolyzed gelatin, .alkali -hydmlyzed gelatin, pepsinhydrolyzed gelatin as well as pepsin- hydrolyzed pig skin collagen. The different components of rod - like collagen hydrolyses with molecular weight possibly ranging from I to 100 kDalton can be separated, displayed clearly in their electrophoresis scanned chromatograms and quantitatively characterised successfizlly. In a typical electrophoresls chroraatogram d pepsin hydrolyzed pig skin collagen, all the bands were dearly localised and their molecular weights orderly were shon to be dislributed between 1 and 100 kD after calibrating. All these are of important values foz the test of collagen hydrolyses.%采用凝胶板配方和电泳条件优化后的SDS—PAGE技术对酸法明胶水解物、碱法明胶水解物、胃蛋白酶法明胶水解物、胃蛋白酶法猪皮胶原水解物、明胶以及猪皮胶原蛋白透析物等进行表征，获得了将分布于一块凝胶板上的1～100kD的棒状胶原蛋白各组分的色谱图。胃蛋白酶法猪皮胶原蛋白水解物经电泳分离后的色谱图中，各谱带清晰明显，分子量均一，且均匀分布于1～100kD不等，这对棒状胶原蛋白分子的研究具有重要价值。