响应面分析法优化碱蓬多糖的脱色工艺

摘要

为了优化活性炭对碱蓬多糖的脱色工艺条件,在单因素实验基础上,选取碱蓬多糖脱色过程中的活性炭用量、脱色温度、脱色时间和pH为影响因素,根据Box-Behnken中心组合设计原理,进行响应面法分析,确定碱蓬多糖的最佳脱色工艺条件为:活性炭用量3.3％,脱色温度53℃,脱色时间43 min,pH5.4,脱色率为65.38％,RSD为1.74％.该优化工艺简单、稳定、具有良好的可行性.%The decolorization condition of polysaccharide from Suaeda salsa was optimized using activated carbon.On the basis of single-factor tests,the activated carbon dosage,decolorization temperature,decolorization time and pH of polysaccharide decoloration process were selected as the influencing factors.The decolorization rate of polysaccharide was the response value.The response method of polysaccharide decolorization method was analyzed and according to Box-Behnken center combination design principle.The optimal decolorization conditions of polysaccharide were as follows:the amount of activated carbon was 3.3％,decolorization temperature was 53 ℃,decolorization time was 43 min,pH of 5.4,decolorization rate was 65.38％,RSD was 1.74％.This optimization process was simple and stable with good feasibility.