为寻求生物酶催化废油制备生物柴油的最佳条件,采用响应面法优化其转酯化反应条件。选择不同醇油摩尔比、生物酶添加量、反应温度、反应时间为自变量,生物柴油得率为响应值,利用Box-Benhken中心组合方法进行四因素三水平的实验设计,并做响应面分析,建立回归模型。结果表明,回归方程拟合性好,转酯化反应最优条件为:醇油摩尔比为4∶1,生物酶添加量10%,反应温度为35℃,反应时间为15 h。在此条件下,转酯率为95.9%。%In order to seek the optimal condition for preparing biodiesel from waste oil by biological en-zyme,the condition of transesterification was optimized by response surface methodology.Various molar ratio of methanol to oil,enzyme amount,reaction temperature and reaction time selected as the independ-ent variable,and the yield of biodiesel as response value,the experiment with 4 factors and 3 levels was designed by Box-Benhken central composite,and after data preprocessing a regression model was foun-ded by response surface analysis.Analysis showed that the result of the regression equation obtains good fitting,the optimal condition of the transesterification were:molar ratio of methanol to oil 4∶1 ,enzyme a-mount 10%,reaction temperature 35 ℃ and reaction time 15 h.Under these conditions,the yield of biodiesel was 95 .9%.
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