目的 测定不同采摘时期蓝萼香茶菜中蓝萼甲素的含量,考察最适采收期;研究蓝萼香茶菜中蓝萼甲素的提取工艺.方法 HPLC法,色谱柱为Waters Symmetry C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(70:30),检测波长250 nm,流速1.0 ml/min,柱温35℃,进样量10 μl;单因素试验法比对不同溶剂、不同提取方式对蓝萼甲素提取率的影响,正交试验法优选蓝萼甲素的乙醇提取工艺.结果 7月份采集的蓝萼香茶菜中蓝萼甲素含量最高;甲醇提取的蓝萼甲素含量最高为1.932 mg/g(n=3),95％乙醇次之为1.896mg/g(n=3);乙醇提取蓝萼甲素的最佳工艺为95％乙醇加热回流提取2h,提取1次,固/液比1∶15.结论 蓝萼香茶菜最宜采摘时间为7月份;乙醇提取工艺安全、稳定、成本低,适合工业生产.%Objective To determine the content of Glaucocalyxin A in different period of picking and select the optimum harvest time;to study the extraction process of Glaucocalyxin A from Isodon glaucocalyx (Maxim.) Kudo.Methods The separation is carried out on Waters Symmetry C18 column (4.6 mm × 250 mm,5 μm),the mobile phase composed of methanol and water (70:30),the detection wavelength is set at 250 nm,the flow rate is 1.0 ml/min,the column temperature is 35C and the injection volume is 10 μl;the effect of different solvents and different extraction methods on the extraction rate of Glaucocalyxin A is studied by single factor test,the ethanol extraction process is optimized by orthogonal test.Results In July,the content of Glaucocalyxin A is the highest;Methanol extract have the highest content of Glaucocalyxin A,which is 1.932 mg/g (n =3),95 ％ ethanol is followed by 1.896 mg/g (n =3);the optimum extraction process of ethanol is 95 ％ ethanol,refluxing,extracting 2 h,extracting 1 times,solid / liquid is 1:15.Conclusion Isodon glaucocalyx (Maxim.) Kudo should be collected in July;the process of ethanol extraction is safer,more stable and less expensive,it is suitable for industrial production.
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