Suppression mechanisms on proliferation of glioma U251 cells by FePt nanoparticles through intracellular oxidative stress

摘要

FePt nanoparticles(NPs)have attracted a great deal of attention in biomedical applications due to their excellent superparamagnetic property and anticancer activity,which display promising potential as the MRI/CT contrast imag-ing agent for cancer detection and therapy.In this study,the suppression mechanism induced by FePt NPs on U251 cells is investigated.Glioma U251 cells are co-cultured with FePt NPs at different concentrations,and the intra-cellular oxidative stress was studied by detecting the activities of various redox enzymes and expression of several apoptosis-related proteins.The cell viability assay shows that the highest suppression on the proliferation of glioma U251 cells is 10.5%±5.6%after treatment with 5μg·ml^(-1)FePt NPs for 24 h.In this case,the LDH release remarkably increases.Flow cytometry and fluorescence assay show that FePt NPs promote the apoptosis and G0/G1 phase arrest of U251 cells.