鸽新城疫病毒分离鉴定及动物感染试验

摘要

从发病商品肉鸽组织中分离到3株新城疫病毒，对其基因序列和感染力进行测定和分析。用RT-PCR 对3株病毒 F 基因进行了扩增，测序，3株新城疫病毒的 F 基因同源性比较及进化分析结果表明，3株病毒均属于Ⅵb 亚型，且与2011年比利时分离株亲缘关系较近，F 蛋白裂解位点符合强毒特征；肉鸽攻毒试验进一步证实了3株新城疫病毒的高致病性，肉鸽感染新城疫病毒后通过呼吸道及消化道途径排毒，具有高度接触传染性。试验结果证明了鸽感染新城疫病毒后的排毒时间及方式，为鸽新城疫的及早发现与防控提供了依据。%Three Newcastle disease virus strains were isolated from dead pigeons,the molecular identifica-tion was carried out by RT-PCR method and the F gene was sequenced then.The homology comparison and phylogenetic analysis were also implemented base on the F gene.The result showed that three strains be-long to the Ⅵb subtype.F protein cleavage site was consistent with the virulent NDV strains,which was further verified through pigeon challenge assay.The pigeons infected with NDV shed the virus through the respiratory and digestive tracts showing highly contagious property.The challenge assay demonstrated the shedding way of the infected pigeons which is in favour of the prevention of the pigeon ND.