钝叶瓦松带芽叶片的组织培养和快速繁殖

摘要

The leaves with axillary buds of Orostachys malacophyllus were selected to investigate the effect of different plant growth regulators on axillary buds initiation by using MS medium with different concentrations of 6-benzylamino purine, 1-naphthaleneacetic acid, and 2, 4-dichlorophenoxyacetic acid for developping an efficient procedure of plant regeneration of O. malacophyllus. The results of this study showed that the optimum initiating culture medium for leaves axillary buds of O. malacophyllus was MS medium appended with 3 mg % L % 6-benzylamino purine and 0.1 mg% L 1 1 naphthaleneacetic acid. The optimum proliferative medium for axillary buds of O. malacophyllus was MS medium added with 1. 5 mg. L 1 6-benzylamino purine and 0. 02 mg % L-1 2, 4-dichlorophenoxyacetic acid, and multiplication times was 5.35. In root induction culture, shoot clusters in the1/2 MS medium supplemented with 0. 3 mg % L 1 1-naphthaleneacetic acid were well rooted and grew vigorously with the 96.67% rate of root for- mation, and the best survival rate was 76.67%. This study suggested that the efficient plant regeneration in O. malacophyllus from leaves with axillary buds was possible.%以钝叶瓦松（Orostachys malacophyllus）带芽叶片为外植体,MS为基本培养基添加不同质量浓度的6-BA、NAA和2,4-D,探讨了不同植物生长调节剂对钝叶瓦松带芽叶片启动的影响,以期筛选出最佳培养基配方,建立钝叶瓦松组织培养再生体系。结果表明,叶片腋芽最佳启动培养基为MS＋6-BA 3 mg·L-1＋NAA 0.1mg·L-1,腋芽增殖的最佳培养基为MS＋6-BA 1.5mg·L-1＋2,4-D 0.2mg·L-1,腋芽的增殖倍数为5.35。最佳生根培养基为1/2MS＋NAA 0.3mg·L-1,生根率为96.67%,移栽后成活率为76.67%。