Hordeum brevisubulatum,a salt-tolerant forage grass species in northern China,was studied to explore the impact of thiophanate methyl on the growth and physiological and biochemical characteristics of endophytic fungi within the wild barley.Results showed that the rate of emergence decreased significantly,from 85％ to 72％,after sown seeds were treated with a 500× dilution of thiophanate methyl for 8 h (P＜0.05),and the seedling carrier rate decreased significantly from 100％ to 12％ (P＜0.05).Fungicide treatment reduced plant height and tillers of wild barley,whereas chlorophyll,malondialdehyde,and proline content and catalase,peroxidase,and superoxide enzyme activity were not significantly affected (P＞0.05),C,N,and P content of wild barley decreased by 4.87％,10.99％,and 13.50％,respectively,after fungicide treatment,compared with that in the control group (P＜0.05).Therefore,treatment of seeds with 500× diluted thiophanate methyl for 8 h was an effective method to eliminate fungal endophytes in H.brevisubulatum.%以我国北方优良耐盐牧草野大麦(Hordeum brevisubulatum)为研究对象,进行了杀菌剂甲基托布津对野大麦内生真菌共生体的生长及生理生化影响的研究.结果表明,甲基托布津稀释500倍浸泡种子8h后播种,出苗率显著降低(P＜0.05),从85％降至72％,幼苗带菌率显著降低(P＜0.05),由100％降至12％;杀菌剂处理可降低野大麦的株高、分蘖,但对其叶绿素含量、丙二醛含量、脯氨酸含量、过氧化氢酶活性、过氧化物酶活性和超氧化物酶活性均未产生显著影响(P＞0.05);杀菌剂处理后野大麦的C、N、P含量较蒸馏水浸种对照分别显著降低了4.87％、10.99％、13.50％(P＜0.05).因此,甲基托布津稀释500倍浸泡种子8h为去除野大麦种传内生真菌的有效办法.
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