组织型激肽释放酶对脑缺血大鼠神经生长因子的影响

摘要

Objective To test the effect of tissue kallikrein on ischemia reperfusion rat and nerve growth factor ( NGF ). Methods 36 Wistar rats were randomly assigned into 3 groups:control group,saline group [ NS 2 mL/( kg· d ) ] ,TK group( TK 1 715 ×10-3 U/( kg·d ) ]. The drug was injected after 8 h of ischemia, lasted for 3 d. We assessed improvement of neurological function at 1,3,7 d after cerebral ischemia. The NGF was determined by Immunohisto chemistry staining. Results Significant recovery of neurological function was found in rats treated with TK comparing with the other groups at 3 d after ischemia( P < 0. 05 ), the expression of NGF increased. Conclusion TK has thera peutical effect on brain ischemia by increasing NGF,and promote nerve regeneration of cerebral ischemia zone.%目的 探讨组织型激肽释放酶(Tissue kallikrein,TK)治疗大鼠缺血性脑梗死的疗效以及对神经生长因子(NGF)的影响.方法 将 36只Wistar大鼠随机分配到3组:空白对照组、盐水对照组[NS 2 mL/(kg·d)]、TK组[TK 1 715×10-3 U/(kg·d)].药物在缺血后8 h给予,连续用药3 d.大鼠梗死后1、3及7 d后分别进行神经功能缺失评分,对脑缺血组织NGF进行免疫组化测定.结果 治疗3 d后,与盐水对照组及空白对照组相比,TK组神经功能缺损明显减轻(P<0.05),缺血区内NGF表达增多(P<0.05).结论 TK可以通过增加NGF而治疗大鼠缺血性脑梗死,促进神经细胞再生.