黄芪对喉癌细胞增殖和凋亡的影响

摘要

Objective To investigate the mechanism underlying the anticancer activity of astragalus on human laryngeal cancer. Methods Hep-2 cells were treated with different concentrations of astragalus for 24 h. MTT assay was used to evaluate cell proliferation. Flow cytometry with PI staining and fluorescent microscopy with hoechst 33258 staining were used to estimate cell cycle distribution and cell apoptosis. Results Astragalus inhibited cellular proliferation in a dose dependent manner( P <0. 05 ). Flow cytometry analysis showed that treatment with astragalus resulted in accumulation of cells at the G2/M phase of the cell cycle and cell apoptosis in a dose dependent manneK P < 0. 05 ). Marked apoptotic changes were observed by 33258 staining. Conclusion Astragalus inhibited cell proliferation and induced apoptosis of Hep-2 cells by regulating cell cycle, forming G2/M phase inhibition.%目的 体外观察黄芪对人喉癌细胞系Hep-2的抑制增殖和诱导凋亡作用并探讨其作用机制.方法 用20、100、200 μg/mL的黄芪作用于Hep-2细胞24 h,MTT法检测细胞抑制率,流式细胞仪检测细胞凋亡率,光学显微镜观察细胞形态学变化.结果 MTT结果显示,黄芪可显著抑制Hep-2细胞增殖且存在剂量依赖性;流式细胞仪检测发现,细胞凋亡率随着黄芪浓度增高逐渐升高,各实验组之间及其与对照组之间比较差异有统计学意义(P<0.05);用药后光镜观察细胞数量减少,荧光显微镜观察可见典型细胞凋亡.结论 黄芪可调控喉癌细胞周期,形成G2/M期阻滞,通过抑制增殖和促进凋亡发挥其抗癌作用.