目的 建立HPLC法同时测定杏花中阿魏酸、芦丁和异槲皮苷.方法 色谱柱为YMC-Pack ODS-A C18柱(250 mm×4.6 mm,5 μm);流动相为乙腈-0.1%磷酸溶液(15∶85);体积流量1.0 mL/min;检测波长为343 nm;柱温为30 ℃.结果 阿魏酸、芦丁和异槲皮苷分别在0.029 08~0.290 8 μg (r=0.999 9)、0.548 4~5.484 2 μg (r=0.999 9)、0.033 07~0.330 7 μg (r=0.999 9)范围内线性关系良好;平均回收率分别为98.31%、98.93%、100.25%,RSD分别为1.0%、0.9%、1.1%.10批杏花样品中阿魏酸、芦丁、异槲皮苷含量测定结果分别为0.105~0.554、3.080~6.933、0.258~0.974 mg/g.结论 该方法多种成分同时测定,操作简便、准确,重复性好,可用于杏花药材的质量控制.%Objective To establish an HPLC method for the determination of ferulic acid,rutin and lsoquercitrin in prunus armeniaca flos.Methods A YMC-Pack ODS-A C18 (250 mm×4.6 mm,5 μm) column was adopted.The mobile phase consisted of acetonitrile-0.1% phosphoric acid solution at a flow rate of 1.0 mL/min.The detection wavelength was 343 nm and the column temperature was 30 ℃.Results The standard curve of ferulic acid,rutin and lsoquercitrin showed a good linearity in the concentration range of 0.029 08~0.290 8 μg (r=0.999 9),0.548 4~5.484 2 μg (r=0.999 9),0.033 07~0.330 7 μg (r=0.999 9),and the average recoveries were 98.31%,98.93% and 100.25%,and RSD were 1.0%,0.9% and 1.1%.The content of ferulic acid,rutin and lsoquercitrin were 0.105~0.554,3.080~6.933 and 0.258~0.974 mg/g,respectively,in 10 samples of prunus armeniaca flos.Conclusion This method is simple,accurate,reproducible and convenient for the quality control of prunus armeniaca flos.
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