Objective:To study the applicability of Demirjian′s method for age estimation in children of Nanjing .Methods: 644 panoramic radiographs(378 boys and 266 girls) which were collected from Affiliated Hospital of Stomatology of Nanjing Medical Uni-versity were involved in this study with chronological age from 3 to 16 years.Paired t-test was performed between the chronological age and the age determined by Demirjian′s method.Single-factor model was used to explore the relationships of the two kinds of age .Re-sults:For the marked difference between the chronological age and determined age (P<0.01),Demirjian′s method was not suitable to the children in Nanjing.We set up the mathematical model between tooth maturity and natural age of children in Nanjing ,then got the curve-fitting equation.For boys:Y=43.37Sin(0.02579X-1.138) +30.6SIN(0.04275X +0.9984) +3.019SIN(0.08706X +1.649),and for girls:Y=55.93SIN(0.04001X-1.642)+90.97SIN(0.05389X+0.7095) +44.25SIN(0.06254X+3.384).The Results of paired t-test between chronological age and predicted age from quadratic equation showed that there was no significant differ -ence in boys and girls(P>0.05).Conclusions:Through the establishment of mathematical model between tooth maturity and natural age in this study,we can determine the natural age from 3 to 16 in Nanjing children more accurately by utilizing tooth maturity .% 目的：研究Demirjian法（下文简称“D法”）推断南京地区儿童自然年龄的适用性。方法：收集南京医科大学附属口腔医院符合纳入标准的年龄在3～16岁患者的全口曲面断层片共644例，其中男性378例，女性266例。按照D法进行牙龄推断，所得牙龄与自然年龄进行配对t检验，通过建立单因素数学模型探索两者之间的对应关系。结果：直接运用D法对南京地区儿童进行牙龄推断，所得出的结果与实际年龄相比，差异有统计学意义（P＜0．01）。建立南京地区儿童牙齿成熟度与自然年龄间的数学模型，得到自然年龄与牙总成熟度的拟合曲线方程：男子组为Y＝43．37SIN（0．02579X－1．138）＋30．6SIN （0．04275X＋0．9984）＋3．019SIN（0．08706X＋1．649），女子组为Y＝55．93SIN（0．04001X－1．642）＋90．97SIN（0．05389X＋0．7095）＋44．25SIN（0．06254X＋3．384）。将其推断的年龄与实际自然年龄比较，两者间的差异无统计学意义（P＞0．05）。结论：本研究通过建立儿童牙齿成熟度与自然年龄间的数学模型，可以利用牙齿发育程度较为准确推断南京地区3～16岁儿童的自然年龄。
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