STIM1在口腔扁平苔藓患者中表达的研究

摘要

Objective:By studying the expression of stromal interaction molecule 1 (STIM1 ) in lesions of oral lichen planus (OLP)to explore the pathogenesis of OLP.Methods:The mRNA expression level of STIM1 in 37 OLP and 17 healthy adults′peripher-al blood lymphocytes was determined by real-time fluorescence quantitative PCR.The protein expression level of STIM1 from the same populations was determined by Western blot.Results:Increased STIM1 mRNA expression was found in OLP to be higher than that in healthy populations (P<0.01);Expression of STIM1 protein was significantly higher in OLP than in healthy controls (P<0.01). Differences between two groups of OLP patients were not statistically significant (P >0.05 ).Conclusions:Increased expressions of STIM1 in OLP peripheral blood lymphocytes,which can regulate lymphocyte immune response,may be involved in the pathogenesis of OLP and play an important role in the process of repeated episodes of OLP.%目的：通过观察基质相互作用分子1（stromal interaction molecule 1，STIM1）在口腔扁平苔藓（oral lichen planus，OLP）患者外周血淋巴细胞中的表达，探索 OLP的发病机制。方法：采用实时定量 RT-PCR的方法，检测37例 OLP 患者及17例健康成人外周血淋巴细胞中的 STIM1基因表达水平；采用 Western blot法检测上述对象外周血淋巴细胞中的 STIM1蛋白表达水平。结果：糜烂型和非糜烂型 OLP患者中 STIM1基因和蛋白的表达水平均高于对照组，且差异有统计学意义（P＜0．01）；但糜烂型和非糜烂型 OLP患者组间表达的差异均无统计学意义（P＞0．05）。结论：STIM1在 OLP 外周血淋巴细胞中高表达，通过对淋巴细胞的免疫调控参与了 OLP的发病过程。