法尼醇对不同状态白念珠菌氟康唑药物敏感性作用的比较研究

摘要

Objective:To explore the effect of farnesol on sensitivity to fluconazole of Candida albicans ( C.albicans) in different states.Methods:We used XTT reduction assay to test the inhibitory effects of farnesol on the C.albicans planktonic and biofilms in vitro.The research included four groups:farensol-treated planktonic group, farnesol-free planktonic group, farnesol-treated biofilms group and farnesol-free biofilms group.Results:The XTT analysis suggested there is no significant difference on the antifungal susce-pitibility of farnesol-treated planktonic and farnesol-free planktonic when treated with fluconazole (P>0.05), but there is remarkable difference on farnesol-treated biofilm and farnesol-free biofilm when treated with flucoazole (P<0.05).Conclusions:Farnesol can in-crease the antifungal suscepitibility of C.albicans biofilm.%目的：探讨法尼醇处理对不同生长状态白念珠菌的氟康唑药物敏感性的影响。方法：体外构建浮游状态白念珠菌和成熟生物膜状态白念珠菌；法尼醇处理后，用甲基四氮盐（ XTT）法检测浮游状态和生物膜状态白念珠菌细胞活性的改变。结果：与对照组比较，法尼醇处理后的浮游状态白念珠菌对氟康唑的敏感性无明显改变（P＞0．05），而生物膜状态白念珠菌对氟康唑的敏感性增高（P＜0．05）。结论：法尼醇可以增加白念珠菌生物膜对药物的敏感性。