两种多糖作为迟缓爱德华氏菌（Edwardsiella tarda）灭活疫苗佐剂对大菱鲆（Scophthalmus maximus）的免疫保护效果

摘要

The immune protection effects of two polysaccharides as the adjuvants of inactivated Edwardsiella tarda vaccine in turbot were studied in this research. The vaccines were prepared with two polysaccharides, astragalus polysac- charides (APS) and glucan (Glu) were added to the inactivated E. tarda vaccine separately. Turbots Scophthalmus maximus were inoculated by intraperitoneal (i.p.) injection. The lysozyme activity (Lyz), superoxide dismutase activity (SOD), and antibody titer in the sera were determined after 28 day post-vaccination; meanwhile, the relative percentage survival (RPS) in each group was compared. The results indicated that the addition of polysaccharide adjuvants in the inactivated vaccine could enhance the immune functions to the vaccinated turbots. The Lyz, SOD, and the antibody titer in the sera of the tur- bots inoculated with the mixtures of inactivated vaccine and each polysaccharide adjuvant were significantly increased (P〈0.05). The RPS in the vaccination group with the mixture of inactivated vaccine and 2.5mg/ml APS and the vaccination group with the mixture of inactivated vaccine and 5mg/ml Glu have reached to the best protection effects at (78.7±1.3)% and (64.0±8.9)%, respectively. The SOD, Lyz, and the antibody titer in the best RPS groups with the two polysaccharides also reached higher levels than those in the other groups did.%采用黄芪多糖、葡聚糖作为免疫佐剂，与迟缓爱德华氏菌灭活疫苗配伍后注射免疫大菱鲆，测定免疫28d后血清中溶菌酶活力、SOD活力、抗体效价和各免疫组的相对保护率（RPS）。结果表明，添加多糖免疫佐剂能提高疫苗免疫的大菱鲆的各免疫指标，添加佐剂的免疫组的血清溶菌酶活力、SOD活力和血清效价比单纯的疫苗免疫组显著提高（P〈0．05），2．5mg／ml黄芪多糖混合疫苗免疫组和5mg／ml葡聚糖混合疫苗免疫组的相对保护率最高，分别达（78．7±1.3）％和（64．0±8．9）％，且溶菌酶活力、s0D活力及血清效价等指标较其他各组有提高。