于2010年6月,以性状优良的白黄壳内面F2代菲律宾蛤仔二元杂交系（WO）与紫壳内面F2代选育系（P）为材料,进行了三元杂交研究,获得了三元正反交组合PWO、WOP,并且比较了各实验组子代在不同阶段生长、存活和变态的杂种优势。结果表明：（1）从生长上看。三元杂交子WOP生长始终最快,2个三元正反交组幼虫均表现出了不同程度的杂种优势,其生长主要受卵源与配对策略的交互作用影响。（2）从存活力上看。三元杂交子PWO幼虫及WOP稚贝表现出一定的杂种优势,卵源是浮游期幼虫存活的最主要影响因素。（3）从变态上看,三元杂交子PWO呈现出一定的变态优势为HmPWO=9.80,卵源、配对策略及交互作用均是影响变态主要因素。%In order to improve the phenotypic character of Manila clam Ruditapes philippinarum, three way crosses were conducted between the generation F2 of purple （metamorphic heterosis） and white-orange （growth and survival heterosis） inner-shell color of R. philippinarum in June 2010. The growth, heterosis of reciprocal crosses （PWO, WOP） was investigated in the study. The results showed that： （1） As to growth, WOP （generation of the three way crosses） was the fastest experimental group during the planktonic period, and two crosses showed their heterosis in different degrees （HgPWO = 4.90±4.17 and HgWOP = 2.12±1.77） . It was mainly affected by the interaction between egg origin and mating strategy, and the next one is egg origin effect during the planktonic stage. During the juvenile stage, WOP was still the fastest one in growth, remarkably different with other groups （P0.05） and showed obvious heterosis （HgWOP = 14.87±4.04）. （2） As to survival rate, PP （self-fertilized group） was the lowest, and PWO （generation of the three way crosses） showed survival heterosis （HsPWO = 7.26±6.93） during the planktonic period. It was mainly affected by egg origin in planktonic survival. During the juvenile stage, WOP have a higher survival ability, and showed a little heterosis （HsWOP = 2.09±3.42）. （3） As to metamorphosis, PP showed the lowest metamorphic ratio, it was remarkably different with other groups （P0.05）. The other three groups made no remarkable difference each other （P0.05） , and the heterosis of PWO was 9.80.
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