黄花水龙化感物质对铜绿微囊藻生长及藻毒素产生和释放的影响

摘要

The inhibition of allelochemicals extracted from Jussiaea stipulacea Ohwi on the growth of Microcystis aeruginosa and the optimal dose range for allelochemicals' anti-algal effect were investigated,and their impact on production and release of microcystin-LR (MC-LR) tested.The experimental results indicate that,the allelochemicals had a significant inhibitory effect on the algae growth and the half-effect concentration was 47mg/L within 7 days of culture.However,the inhibition declined with the cultivation time.The allelochemicals showed the maximum inhibitory effect on the algae growth in dose range 50-75mg/L; and the relative inhibitory ratio (IR％) reached 50％-95％ in algae cell cultures in 4-7 days.In addition,the extracted allelochemical compounds demonstrated no significant impact on the extracellular release of MC-LR during the whole culture period.The amount of intracellular MC-LR per 106algal cells increased dependent on the increasing dose of the allelochemicals after 7 days of culture and then stayed after 16 days.There was no increase in the total amount of MC-LR in the algal cell culture medium.Therefore,applying the allelochemicals extracted to inhibit M.aeruginosa is a good practice and ecologically safe for treating water in algae bloom.The treatment can inhibit the proliferation of algal cells effectively without generating cyanotoxin.%本文研究了黄花水龙化感物质对铜绿微囊藻生长的抑制作用及其最佳抑藻浓度范围,以及对微囊藻毒素(MC-LR)产生和释放的影响.结果表明:黄花水龙化感物质在培养7d内对铜绿微囊藻(FACHB-905)具有较强的抑制作用,半效应质量浓度(EC50,7d)值为47mg/L,但抑制效果会随时间的延长而减弱.黄花水龙化感物质的最佳抑藻浓度范围为50-75mg/L,藻细胞培养4-7d内相对抑制率达50％-95％.同时发现整个培养期间,黄花水龙化感物质对MC-LR的胞外释放无显著影响.培养7d后,单位藻细胞内MC-LR的含量随黄花水龙化感物质浓度的增加而升高,16d后无显著影响.藻细胞培养液中MC-LR总量不会增大.由此可知,利用黄花水龙化感物质抑制铜绿微囊藻,既能有效控制藻细胞生长又不会促进藻毒素的释放,生态安全性高,可应用至实际水体水华处理中.