Two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry for detection of protein expression profiles in the hippocampus following closed brain injury

摘要

Gene expression profile changes in brain regions following traumatic brain injury at the gene level cannot sufficiently elucidate gene expression time,expression amount,protein post-translational processing or modification.Therefore,it is necessary to quantitatively analyze the gene expression profile using proteomic techniques.In the present study,we established a rat model of closed brain injury using Marmarou's weight-drop device,and investigated hippocampal differential protein expression using two-dimensional gel electrophoresis and surface-enhanced laser desorption ionization-time of flight-mass spectrometry.A total of 364 protein peaks were detected on weak cation exchange-2 protein chips,including 37 differential protein peaks.345 protein peaks were detected on immobilized metal affinity capture arrays-Cu,including 12 differential protein peaks Further examination of these differential proteins revealed that glucose-regulated protein and proteasome subunit alpha type 3 expression were significantly upregulated post-injury.These results indicate that brain injury can alter protein expression in the hippocampus,and that glucose-regulated protein and proteasome subunit alpha type 3 are closely associated with the occurrence and development of traumatic brain injury.