Expression and subcellular localization of cysteine- and glycine-rich protein-2 in rat undifferentiated olfactory stem cells

摘要

BACKGROUND: As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 (CRP2) has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previous study has demonstrated that CRP2 can be detected in the embryonic rat inner ear but not in the adult rat inner ear. However, at present, the expression of LIM protein family Ⅱ members in stem or precursor cells has not been described. OBJECTIVE: To determine the expression and sub-cellular localization of CRP2 in olfactory stem cells. DESIGN, TIME AND SETTING: An experiment with repeated measures was performed in the Laboratory of Otorhinolaryngology, Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University from February 2008 to April 2008. MATERIALS: Olfactory stem cells, and rabbit-anti-CRP2 polyclonal antibody were prepared and kept in our laboratory. METHODS: Reverse transcription polymerase chain reaction and Western blot analysis were used to detect expression of CRP2 in olfactory stem cells. Immunocytochemistry was also used to localize CRP2 in olfac- tory stem cells. MAIN OUTCOME MEASURES: The expression and sub-cellular localization of CRP2 in rat olfactory stem cells. RESULTS: CRP2 expression was found in olfactory stem cells, and CRP2 was distributed in both the nu- cleus and the cytoplasm. CONCLUSION: Confirmation of the expression and distribution of CRP2 in olfactory stem cells.