不同产地黑老虎果实不同部位抗氧成分及抗氧化活性比较

摘要

经超声波提取测定了不同地区(湖南、江西和贵州)黑老虎果实不同部位(果皮,果肉和籽)的抗氧化活性成分及抗氧化活性.结果表明,贵州地区黑老虎抗氧化活性成分多酚、黄酮、花色苷、维生素C含量最高、抗氧化能力最强,湖南地区黑老虎抗氧化活性成分含量最低.三个产地黑老虎抗氧化活性成分含量均为果皮＞果肉＞耔.贵州黑老虎果皮的多酚含量(TPC)、黄酮含量(TFC)、花色苷含量(TAC)、维生素C分别达3.155±0.006 mg GAE/g FW、12.685±0.041 mg CE/g FW、0.635士0.013 mg CYE/g FW、87.591士0.082 mg VC/g FW.其DPPH自由基清除能力、ABTS自由基清除能力和铁还原抗氧化能力FRAP值分别达287.527士0.384 μmol Trolox/g、208,468士1,072μmol Trolox/g和200.878± 1.224μmol Trolox/g.Spearman相关性研究表明,多酚、黄酮、花色苷、维生素C含量与抗氧化活性呈强相关.研究结果为黑老虎的深加工利用提供了理论依据.%Different parts of Kadsura coccinea from Hunan,Jiangxi,Guizhou were extracted by ultrasonicassited extraction,and the antioxidant contents and antioxidant activities in the extracts of peel,pulp and seed were determined and compared.The results showed that the highest content of antioxidant substances such as polyphenols,total flavonoids,VC and anthocyanin in the Kadsura coccinea was found in Guizhou,while the lowest level was found in Hunan area,these contents of antioxidant substances in three area were all showing the same tendency,that is in peel ＞in pulp ＞in seed.The contents of polyphenols,flavonoids,anthocyanins and vitamin C reached to 3.155±0.006 mg GAE/g FW,12.685±0.041 mg CE/g FW,0.635±0.013 mg CYE/g FW,and 87.591±0.082mg VC/g respectively,and the DPPH,ABTS,FRAP antioxidant activities in Peel of Kadsura coccinea from Guizhou are 287.527±0.384 μmol Trolox/g FW,208.468a1.072 μmol Trolox/g FW and 200.878±1.224 μmol Trolox/g FW,respectively.Spearman correlation study showed that the content of polyphenols,flavonoids,anthocyanins and vitamin C is strongly related to antioxidant activity.The research provides a theoretical basis for the deep processing of Kadsura coccinea.