穴位注射胞二磷胆碱对创伤性脑损伤大鼠血清神经元烯醇化酶、S100-β蛋白的影响

摘要

Objective It is tO investigate the effects Of injectiOn Of citicOline intO Zusanli pOint On the cOntents Of serum neurOn specific enOlase( NSE)and S-100β prOtein in the rats with traumatic brain injury. Methods Thirty healthy adult male Sprague-Dawley( SD)rats were randOmly divided intO five grOups:sham-Operated grOup( grOup A),acupuncture pOint drug injectiOn grOup(grOup B),acupuncture pOint saline injectiOn grOup(grOup C),intraperitOneal drug injectiOn grOup(grOup D)and cOntrOl grOup( grOup E). Opened brain trauma was induced by the imprOved Feeney methOd in the 24 rats Of grOups B,C,D and E. Six sham-Operated rats were subjected tO the same surgical prOcedure,except brain injury. The rats in grOups B and C were treated with acupuncture injectiOn Of citieOline Or saline respectively. The rats in grOups D and E were treated with intraperitOnea injectiOn Of citicOline Or saline respectively. Serum NSE and S-100β prOtein were determined at 24 hOurs after mOdel established. Results The cOntents Of NSE and S-100β in B,C,D,E grOups were mOre than that Of grOup A( P <0. 05),while the cOntent Of NSE and S-100β in grOup B was lOwer than in C,D,E grOups(P<0. 05). Conclusion Acupunc-ture pOint drug injectiOn therapy might reduce the cOntents Of S-100β and NSE and prOvide ObviOus prOtectiOn effect fOr trau-matic brain injury.%目的：观察足三里穴位注射胞二磷胆碱疗法对创伤性脑损伤大鼠血清神经元烯醇化酶（ NSE）、S100-β蛋白含量的影响。方法成年健康雄性 SD 大鼠30只，采用改进的 Feeney法建立大鼠脑损伤模型，设假手术组大鼠6只（ A 组），造模成功大鼠24只随机分为穴位注药组（B组）、穴位注水组（C 组）、腹腔给药组（D 组）和腹腔注水组（E 组）各6只。造模后1 h，B、C、D、E组分别单次给予足三里穴位注射胞二磷胆碱或生理盐水，腹腔注射胞二磷胆碱或生理盐水处理。造模后24 h检测各组大鼠血清NSE和S100-β含量。结果 B、C、D、E各造模组与 A组比较，S-100β和 NES 含量均显著升高（P 均＜0.05）。在造模各组中，B组 S-100β和 NES含量明显低于 C、D、E 组（P 均＜0.05）。结论穴位注射胞二磷胆碱疗法可减少创伤性脑损伤大鼠 S-100β和 NES的产生，对创伤性脑损伤有一定的神经保护作用。