多通道高通量DNA合成仪控制系统设计与实现

摘要

Objective To establish an auto-control system for high-throughput and multi-channel DNA synthesis which can simultaneously and quickly synthesize up to 96 different oligonucleotides in a 96-well microtiter format.Methods The PLC and its extended modules is used as the main-control unit, which executes the DNA automatic synthesis process according to the synthesis sequences and steps set by the user,and the manual injecting reagent etc.And the configuration software and VC6.0 were used for programming the man-machine interface sofeware to set synthesis parameters, position calibration,flux calibration data etc, and communicated with PLC.Results The synthesis application of about 150 000 DNA chains has proved that the synthesis cycle time for 96 couplings was 4 min,the average coupling efficiency was 99%across the entire 96-well plate,the monomer reagent usage was reduced by 50 percent,and the synthesis configuration was more flexible.Conclusion A reliable and simple auto-control system is provided for parallel synthesis of 96-channel oligonucleotide chains,which can meet the demands of high-throughput and multi-channel DNA synthesis.%目的：研究一种多通道、高通量DNA合成控制系统，可实现单次96通道寡核苷酸长链的全自动合成。方法该控制系统以松下FP2系列PLC及其外围功能模块为核心，完成合成文件和序列文件的解析执行，以及自动和手动合成操作的过程控制；基于组态软件和VC6．0开发上位机人机交互界面，完成合成过程配置，以及流量校准、位置校准等参数设置，并与主控系统完成数据交互。结果经15万条DNA应用合成及测序，结果表明合成时间达到4 min／cycle，耦合效率为99％，且单体试剂消耗量减少＞50％，合成过程配置灵活。结论 DNA合成仪控制系统可同时实现96个独立通道的寡核苷酸长链的合成，为多通道高通量DNA合成提供了一种高效、灵活、可靠的控制方法。