量子点荧光探针用于汉坦病毒重组抗原的检测

摘要

Objective To detect recombinant Hantavirus antigens by using a quantum dots ( QDs) fluorescent probe. Methods The probe of water-solubility QDs fluorescence nanoparticle was synthesized and modified with protein G and anti-HV Antibodies. We detected recombinant Hantavirus antigens by probe combining immune method,and optimized the detecting conditions. Results The optimum reaction time,pH and goat antibodies concentration for conjugating the QDs were 2 h ,6.0, and 20 μg/ml, respectively. The limit of detection of recombinant antigens was 5 ng/ml. Conclusion The fluorescent probe could effectively recognize HV antigens. The method was specific, sensitive and convenient. We developed a new method for HV antigens detection.%目的 应用量子点荧光探针对汉坦病毒(Hantavirus,HV)重组抗原进行检测.方法 合成水溶性量子点荧光纳米颗粒,并在其表面修饰G蛋白和anti-HV抗体作为量子点荧光探针,对HV重组抗原进行检测并优化检测条件.结果 量子点与抗体的最佳偶联条件:pH 6.0、反应时间2h、抗体浓度为20 μg/ml.用本方法检测HV重组抗原的最低检测值为5 ng/ml.结论 该探针能有效的识别HV抗原,且操作简便快速,为HV重组抗原的检测和肾出血热综合征的诊断提供了新方法.