基于第二代测序技术的细菌基因组与转录组研究策略简介

摘要

随着基于第二代测序技术的细菌基因组与转录组研究越来越广泛,选择合适的研究策略变得越来越重要.就基于第二代测序技术的细菌基因组和转录组研究策略进行综述,并简要介绍细菌基因组和转录组研究中的机遇和挑战.综述细菌基因组与转录组研究的常规方法及步骤,并简要地介绍存在的问题.细菌基因组和转录组研究策略为大多数细菌的研究提供了一个相对完整的研究路线,同时也会促进其它领域的研究,如生命形成、生物进化、基础代谢、疾病、药物等.%Recently, many bacterial genome and transcriptome studies have been performed based on the next-generation sequencing technologies. Therefore, how to select the appropriate research strategies is becoming increasingly important. In this paper, we discussed the research strategies of bacterial genome and transcriptome based on the next-generation sequencing technologies, and stated the oppor-tunities and challenges in these fields briefly. We reviewed the conventional methods and procedures and presented the existing problems briefly for bacteria] genome and transcriptome studies. The research strategies of bacterial genome and transcriptome provide a relatively complete pipeline for the majority of bacteria. Moreover, it will promote the research of other fields, such as the course of life, biological evolution, basal metabolism, disease and drugs.