红树林植物促生菌SZ7-1菌株的培养基优化

摘要

With the applying of completely mixed biological process, microbiological method of removing SO2 was studied as pre-acidified waste molasses was used as carbon source. Under extensive experimental condition, Desulfovibrio desulfuricans' utilization situation of organic acids in pre-acidified waste molasses as well as its desulfurization effect of sulfur dioxide in high concentration were researched, at the same time, the removal rate of the product H2S in the second biological reactor was also determined.The results suggested that Desulfovibrio desulfuricans could exploit pyruvic acid and the lactic acid in pre-acidified waste molasses as its carbon source, with acetic acid as main product. When the inlet concentration of SO2 ranged from 1 865 mg/m3 to 4 637 mg/m3, the removal rate of SO2 was more than 91％ in 1# biological reactor and the final removal rate of SO2 was 95.5％, the produced H2S was nearly all transformed with the mean removal rate of 98％. pH and the concentration of the bacteria were quite stable and the system operated well.%采用响应面分析法对红树林植物促生菌SZ7-1菌株发酵培养基进行了优化.首先利用Plackett-Burman(PB)设计对影响SZ7-1生长的11个营养因素进行评价,并筛选出显著影响因子:玉米糖浆、酵母膏和K2HPO4;其次用最陡爬坡实验逼近以上三因素最优水平;最后采用响应面法对该3个显著因素的最佳水平范围进行研究,得到的最佳浓度为:玉米浆28 g/L、酵母膏14 g/L和K2HPO4 2.2 g/L.在此条件下,培养20 h后SZ7-1菌数达到2.6×1010 CFU/mL,与优化前基础培养基、LB和牛肉膏蛋白胨培养基中生长的菌数相比,分别提高了12.4、2.4和5.5倍.