Correlation of intestinal flora disorder with systemic inflammatory response and stress response in children with severe pneumonia

摘要

cqvip:Objective:To study the correlation of intestinal flora disorder with systemic inflammatory response and stress response in children with severe pneumonia.Methods: The children who were diagnosed with severe pneumonia in Xiangyang No. 1 People's Hospital between April 2014 and December 2017 were selected as the pneumonia group of the study, and the healthy children who received physical examination in Xiangyang No. 1 People's Hospital during the same period were selected as the control group. The feces was collected to determine the number of intestinal flora bifidobacteria and Escherichia coli (E. coli). Besides, the serum was collected to determine the contents of inflammatory cytokines and oxidative stress indexes, and the peripheral blood was collected to determine the expression intensity of inflammatory molecules and oxidative stress molecules.Results:The number of bifidobacteria and the level of Bifidobacterium and E. coli ratio B/E in feces as well as SOD content in serum of pneumonia group were significantly lower than those of control group whereas the number of E. coli in feces, TLR2, TLR4, NOX2, iNOS and FOXP3 expression intensity in peripheral blood as well as G-CSF, sTREM1, TNF-α, LPO and NO contents in serum were significantly higher than those of control group;Pearson correlation analysis showed that B/E level in feces of pneumonia group was negatively correlated with TLR2, TLR4, NOX2, iNOS and FOXP3 expression intensity in peripheral blood as well as G-CSF, sTREM1, TNF-α, LPO and NO contents in serum, and positively correlated with SOD content in serum.Conclusion:The intestinal flora disorder in children with severe pneumonia can aggravate the degree of systemic inflammatory response and stress response in the course of disease.