cqvip:Objective: To study the effect of metformin combined with cisplatin on the expression of genes related to ovarian cancer cell growth and invasion in vitro. Methods: SKOV3 ovarian cancer cell lines were cultured and randomly divided into the control group treated with serum-free DMEM, the DDP group treated with serum-free DMEM containing 10 μmol/L cisplatin, the MET group treated with serum-free DMEM containing 10 mmol/L metformin and the DDP+MET group treated with serum-free DMEM containing 10 μmol/L cisplatin and 10 mmol/L metformin. Results: 24 h after treatment, GRP78, TBX2, CyclinD1, Ki-67, MTA1, CTHRC1, Slug and MMP2 protein expression in DDP group, MET group and DDP+MET group were significantly lower than those in control group whereas Fn14, NDRG2, SOCS1, Beclin1 and ST7L protein expression were significantly higher than those of control group;GRP78, TBX2, CyclinD1, Ki-67, MTA1, CTHRC1, Slug and MMP2 protein expression in DDP+MET group were significantly lower than those in DDP group and MET group whereas Fn14, NDRG2, SOCS1, Beclin1 and ST7L protein expression were significantly higher than those in DDP group and MET group. Conclusion: metformin combined with cisplatin can inhibit the expression of pro-proliferation and invasion genes and promote the expression of pro-apoptosis genes in ovarian cancer cells so as to inhibit cancer cell growth and invasion.
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