Sunitinib inhibits renal cancer cell growth and invasion in vitro through Wnt/β-catenin signaling pathway: an experimental study

摘要

Objective:To study the effect of sunitinib on renal cancer cell growth and invasion in vitro as well as Wnt/β-catenin signaling pathway.Methods: Renal cancer cell lines ACHN were cultured and processed with different doses of sunitinib (1 μmol/L, 2 μmol/L, 4 μmol/L and 8 μmol/L), and sunitinib-free processing condition was used as negative control. 24 h after processing, the mRNA expression levels of apoptosis genes, invasion genes and Wnt/β-catenin signaling pathways in cells were detected.Results: 24 h after treatment, NPRL2, Bax, caspase-3 and caspase-9 mRNA expression in 1 μmol/L, 2 μmol/L, 4 μmol/L and 8 μmol/L sunitinib groups were significantly higher than those in negative control group while MMP2, MMP9, Vimentin, N-cadherin, Wnt andβ-catenin mRNA expression were significantly lower than those in negative control group;the higher the dose of sunitinib, the higher the NPRL2, Bax, caspase-3 and caspase-9 mRNA expression while the lower the MMP2, MMP9, Vimentin, N-cadherin, Wnt andβ-catenin mRNA expression in cells.Conclusion: Sunitinib can inhibit the Wnt/β-catenin signaling pathway in renal cancer cells to increase the expression of apoptosis genes, inhibit the expression of invasion genes and thereby inhibit the cell growth and invasion.