未成熟猪心肌深低温低流量体外循环模型的建立

摘要

Objective To establish the piglets model under deep hypothermia and low flow cardiopul-monary bypass( CPB ). Methods Canmilation of the ascending aorta and the inferior and superior vena cava was performed to establish CPB model. When the nasopharyngeal temperature lowered to 32 ℃, ,the ascending aorta was damped,all animals cooled to nasopharyngeal temperature of 20-25 ℃, ,then they underwent low ilow periusion, and then declamped ascending aorta after 2 hours, rewarming and weaning from the CPB, the changes of the hemodynamics were observed for 2 hours. Results 20 piglets had heart successfully restored beating. The mean arterial pressure and heart rate were not significantly different before CPB and after CPB ( P ＞0. 05 ). The difference oi the hematocrit during CPB was statistically significant( P ＜0. 05 ). The blood gas analysis was normal after adjustment. Conclusion This experiment shows that piglet CPB model is more suitable ior the clinical research.%目的 探索建立未成熟猪深低温低流量体外循环(CPB)模型的实验方法.方法 采用升主动脉-上下腔静脉插管建立未成熟猪CPB模型,待鼻咽温降至32 ℃阻断升主动脉,继续降温至鼻咽温20 ～25 ℃低流量持续灌注,阻断2 h后开放,进行复温、调整停机,停机后观察血流动力学指标2 h.结果 停机后20只乳猪均能顺利复跳,平均动脉压、心率在CPB前后比较差异均无统计学意义(P＞0.05).各时间点血细胞比容比较差异有统计学意义(P＜0.05),各项血气指标经调整都在正常范围之内.结论 未成熟猪CPB模型是更适合临床研究CPB的理想动物模型.