目的 建立颅咽管瘤(Craniopharyngioma,CP)的细胞体系,观察、研究并评估其生物学特点,为在细胞水平研究颅咽管瘤提供实验资料和理论基础.方法 利用特殊培养基对人颅咽管瘤细胞体外原代培养,并进行传代培养,建立肿瘤细胞系,同时观察、检测细胞系的生物学特点.结果 通过消化法并采用特殊的培养基可以成功进行颅咽管瘤细胞的原代培养,通过特殊的培养条件可以使细胞得到纯化并传代培养.原代颅咽管瘤细胞呈多角形,光镜下呈典型的铺路石样表现,胞浆丰富,并可进行传代培养,最多可达8代,免疫细胞化学检测显示角蛋白7阳性.肿瘤细胞生长曲线提示细胞生长稳定,倍增时间约为3天左右.结论 利用CP患者的新鲜肿瘤组织,采用酶消化法并用特殊的培养基进行培养,可以成功地进行颅咽管瘤细胞的原代培养并可在特殊条件下进行传代培养.%Objective To establish cell line of craniopharyngiomas(CP) and to investigate the characteristics of the tumor cell for providing a useful research tool and basing rationale of research on cell level.Methods Human craniopharyngiomas cells were primarily cultured with a special medium in vitro,and the biological characteristics of tumor cells were investigated by cell count and MTT experiment.Results We established the cell line of primary craniopharyngiomas in vitro by the way of digestion culture successfully.Now we got the purified cells and serial subcultivation in cell line.We can see the cells digested by enzyme at the same time if we culture cells by the way of digestion culture,all of them have the morphology of round shape,they became little shuttle shape in the second day.The CP cells have the same shapes approximately,slabstone-like shapes and polygonal shapes.The cell line we found was identified as CP cell line by a series of tests.The growth curve showed that the cell can grow stably,and the doubling time was about 3 days.Conclusion Craniopharyngioma cell line can be cultured with a special medium by enzymatic digestion through the use of fresh tumor tissue in the CP patients.
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