Objective To observe the effects of CpG oligonucleotide( ODN ) on the angiogenesis of subcutaneous implanted tumor of lung carcinoma in the nude mice. Methods The models of subcutaneous implanted tumor in nude mice was established and randomly divided into 4 groups: group A receiving radiation and medical administration[ CpG ODN 1826( 1 μg/μl ) + (3 ray( RT:8 Gy)], group B receiving simple medical administration [ CpG ODN 1826 ( 1 μg/μl)], group C receiving irradiation [ saline ( 100 μl/one ) + (3 ray( RT:8 Gy ) ]and group D[ saline( 100 μl/one ) ]. Seven days after the implantation of lung cancer cell, all those groups received the intratumoral injection of medicine and irradiation for 5 d. The expression of VEGF - C mRNA and NRP - 1 mRNA in the tumor tissue was detected by RT - PCR. Results The relative expression levels of VEGF - C mRNA in the tumor tissue of the four groups above were respectively 18. 34 ± 3. 19,29. 62 ± 3. 14,51. 13 ± 2. 81 and 53. 46 ±5. 67. The relative expression levels of NRP - 1 mRNA in those groups were respectively 23. 57 ± 5.73,34.72 ± 5. 13,59. 95 ±4.76 and 62.49 ± 6. 34. The expression level of group A decreased very significantly compared with those of the other three groups( P <0. 01 ). Compared with the expression levels of group C and group D,that of group B decreased very significantly( P <0. 01 ). Conclusion CpG ODN1826 can remarkably inhibit the angiogenesis of nude mice tumor, and its mechanism may be correlated with the inhibition of VEGF - C and NRP - 1 expression.%目的 观察CpG ODN对裸鼠肺癌皮下种植瘤血管生成的作用.方法 建立裸鼠皮下种植瘤模型,随机分为4组:A组[CpG ODN1826(1μg/μl)+β射线(RT:8 Gy)]、B组[CpG ODN1826(1 μg/μl)]、C组[生理盐水(100 μl/只)+β射线(RT:8 Gy)]、D组[生理盐水(100 μl/只)].接种肺癌细胞7 d后,开始瘤内注射药物并照射5 d.RT-PCR法测定瘤组织中VEGF-C mRNA、NRP-1 mRNA的表达.结果 4组裸鼠肿瘤组织中VEGF-C mRNA相对表达水平分别为18.34±3.19、29.62±3.14、51.13±2.81、53.46±5.67;NRP-1 mRNA相对表达水平分别为23.57±5.73、34.72±5.13、59.95±4.76、62.49±6.34,A组较其他3组显著下降(P＜0.01),B组较C组和D组也显著下降(P＜0.01).结论 CpG ODN1826可显著抑制血管生成,其作用机制可能与抑制VEGF-C和NRP-1的表达有关.
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