归芪益元膏对重离子辐射旁效应损伤大鼠肺肾组织caspase-3及caspase-9表达的影响

摘要

Objective To explore the influence of Guiqiyiyuan Ointment on the expressions of caspase-3 and caspase-9 in the lung and kidney of the rats damaged by heavy ion (12C6+) radiation-induced bystander effect.Methods The Wistar male rats were equally and randomly divided into seven groups,normal control group (NCG),radiation alone group (RAG) and Chinese medicine group (CMG),with the latter two groups being redivided into 6,12 and 24h groups according to the executing time.The Chinese medicine groups were given Guiqiyiyuan Ointment by gavage for two weeks in advance.The normal control group and the radiation alone groups were given the equal normal saline.Afterwards,the right lung of the rats in the radiation alone groups and Chinese medicine groups were radiated by 2Gy 12C6+ ion once.The rats in normal control group were not radiated.All groups of rats were executed 6,12,and 24h after radiation.The protein and mRNA expressions of caspase-3 and caspase-9 in the right lung,left lung and left kidney were examined with immunohistochemistry and Q-PCR.Results Compared with the normal control group,the mRNA expressions of caspase-3 and caspase-9 in the right lung,left lung and left kidney in the radiation alone groups obviously increased 6 and 24h after radiation.While the protein expressions of caspase-3 and caspase-9 in the radiation alone group obviously increased only 24h after radiation (P＜0.01).Compared with the radiation alone groups,the expressions of protein and mRNA ofcaspase-3 and caspase-9 were obviously down-regulated in the Chinese medicine groups (P＜0.01).Conclusion By controlling the up-regulation of the expression ofcaspase-3 and caspase-9,Guiqiyiyuan Ointment can prevent the lung and kidney cell apoptosis and alleviate the damage caused by heavy ion radiation-induced bystander effect in vivo.%目的 探讨归芪益元膏对重离子(12C6+)辐射旁效应损伤大鼠肺肾组织中caspase-3及caspase-9表达的影响.方法 42只雄性Wistar大鼠随机分为7组,即正常对照组(NC组)、单纯辐射组(RO组)、中药组(CM组),后两组按照射后处死时间分为6、12、24h亚组,每组6只.CM组预先用归芪益元膏灌胃2周,NC组和RO组用等量生理盐水灌胃;2周后RO组和CM组大鼠右肺给予2Gy 12C6+离子束单次照射,NC组不照射.于照射后6、12、24h处死各组大鼠,RT-qPCR及免疫组化检测大鼠右肺、左肺、左肾组织中caspase-3、caspase-9mRNA及蛋白的表达.结果 与NC组比较,RO组大鼠右肺、左肺、左肾caspase-3、caspase-9mRNA表达在照射后6、12、24h均明显升高,而蛋白表达仅在照射后24h明显升高,差异有统计学意义(P＜0.01).CM组大鼠右肺、左肺、左肾caspase-3、caspase-9mRNA及蛋白表达较同时间点的RO组明显下调,差异有统计学意义(P＜0.01).结论 归芪益元膏可抑制大鼠肺肾组织caspase-3、caspase-9表达的上调,从而减轻重离子辐射诱导的体内旁效应损伤.