酮咯酸氨丁三醇和罗哌卡因合用对小鼠创口愈合的影响

摘要

Objective:To study the effect of ketorolac tromethamine and ropivacaine on the expression of GM-CSF involved in wound healing.Method:The 128 healthy adult male Kunming mice,cleaning grade, were randomly divided into four groups and group D assigned to be injected intraperitoneally Saline with a equal dose 0.1 mL/(kg·12 h).Group L injected 0.5% Ropivacaine 1 mL/(kg·12 h) around the cut.N group intraperitoneally Ketorolac Tromethamine 5 mg/(kg·12 h).Group H injected Ropivacaine 1 mL/(kg·12 h) around the cut combineing with intraperitoneally Ketorolac Tromethamine 5 mg/(kg·12 h),respectively,in 12 hours.After the completion of the incision in mice models,we randomly selected eight mice without being putted back from every group at the 3rd,the 7th and the 10th day,then measured the expression of GM-CSF by Elisa. The rest 8 mice in the every group would be continued to feed to heal completely in order to record the time that the wound healing takes and observe the intertion status.Result:The expression of GM-CSF between group H and D were compared,group H was higher at 7th ,10th day(P<0.05).The healing time of group H was shorter than that of group D(P<0.05).The area of scar in the group H was smaller than group L(P<0.05),compared with group D,the difference was statistically significant(P<0.05).Conclusion:Ropivacaine combining with Ketorolac Tromethamine can increase the expression of GM-CSF and promote the healing of wound without causing the excessive scar hyperplasia.%目的：研究酮咯酸氨丁三醇和罗哌卡因对创面粒细胞-巨噬细胞集落刺激因子（GM-CSF）的表达和伤口愈合的影响。方法：选取清洁级雄性成年昆明小鼠128只，随机分为四组，D组小鼠腹腔注射等剂量的氯化钠溶液0.1 mL/（kg·12 h）；L组于伤口周围浸润注射0.5%的罗哌卡因1 mL/（kg·12 h）；N组腹腔注射酮咯酸氨丁三醇注射液5 mg/（kg·12 h）；H组腹腔注射酮咯酸氨丁三醇注射5 mg/（kg·12 h）复合创口周围浸润注射0.5%的罗哌卡因1 mL/（kg·12 h）。在小鼠手术切口模型完成后的第3、7、10天，每次不放回地从每组中随机抽取8只小鼠随后用Elisa法测定剪取组织中的GM-CSF浓度，并观测每组剩余8只小鼠的切口愈合时间和情况。结果：H组小鼠在第7、10天时伤口GM-CSF 表达较D组高（P<0.05）；在愈合时间上，H组较D组的愈合时间短（P<0.05）；在愈合后瘢痕面积来看，H组的瘢痕面积较L组小（P<0.05），与D组比较差异也有统计学意义（P<0.05）。结论：酮咯酸氨丁三醇和罗哌卡因合用可促进小鼠创面粒细胞-巨噬细胞集落刺激因子表达，加快伤口的愈合，且不会引起瘢痕的过度增生。