Dynamic full-field optical coherence tomography:3D live-imaging of retinal organoids

摘要

Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information.We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids.Coloured images with an endogenous contrast linked to organelle motility are generated,with submicrometre spatial resolution and millisecond temporal resolution,creating a way to identify specific cell types in living tissue via their function.