Dynamic conjugate F-SHARP microscopy

摘要

Optical microscopy is an indispensable tool in biomedical sciences,but its reach in deep tissues is limited due to aberrations and scattering.This problem can be overcome by wavefront-shaping techniques,albeit at limited fields of view(FOVs).Inspired by astronomical imaging,conjugate wavefront shaping can lead to an increased field of view in microscopy,but this correction is limited to a set depth and cannot be dynamically adapted.Here,we present a conjugate wavefront-shaping scheme based on focus scanning holographic aberration probing(F-SHARP).We combine it with a compact implementation that can be readily adapted to a variety of commercial and home-built two-photon microscopes.We demonstrate the power of the method by imaging with high resolution over extended FOV(>80μm)deeper than 400μm inside a mouse brain through a thinned skull.