P53上游凋亡调节蛋白在依托泊苷诱导直肠癌细胞凋亡中作用机制的研究

摘要

Objective To study the expression and related mechanism of P53 up-regulated modulator of apoptosis (PUMA) with cell apoptosis in human colorectal tumor cell line HCT116. Methods The HCT116 were induced with etoposide. The apoptosis of HCT116 was determined by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. The expressions of PUMA and P53 protein were determined by Western blot. Results Etoposide inhibited the proliferation of HCT116 in time- and dose - independent manners. The expression level of the PUMA and P53 protein increased gradually. Conclusions Etoposide can induce the apoptosis of HCT116 and kill the tumor cells by the expressions of PUMA and P53 protein.%目的 研究P53上游凋亡调节蛋白(PUMA)在直肠癌细胞HCT116中的表达及其关联性作用机制.方法 应用依托泊苷诱导直肠癌细胞HCT116,采用酶联免疫吸附试验(ELISA)、流式细胞仪测定依托泊苷作用前后细胞HCT116的凋亡情况;蛋白印迹法(Western blot)测定PUMA、P53蛋白的表达水平.结果 依托泊苷可抑制直肠癌细胞HCT116生长,并呈时效和量效关系; 依托泊苷作用前后PUMA、P53蛋白表达水平逐渐升高.结论 依托泊苷通过恢复P53的功能增加PUMA的表达,诱导肿瘤细胞调亡,起到抑制肿瘤生长的作用.