早期显性梅毒患者血清IL-2、IL-10和TNF-α检测及其临床意义

摘要

目的 探讨白细胞介素-2(IL-2)、白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)在早期显性梅毒发病机制中的作用.方法 采用双抗体夹心酶联免疫吸附试验(DAbS-ELISA)检测53例早期显性梅毒患者(其中一期梅毒25例、二期梅毒28例)和65名正常人血清IL-2、IL-10和TNF-α水平.结果 早期显性梅毒患者血清IL-2、IL-10和TNF-α水平明显高于正常对照组(P＜0.01)；一期梅毒患者血清IL-2和TNF-α水平明显高于二期梅毒患者(P＜0.01),血清IL-10水平则低于二期梅毒患者(P＜0.01)；早期显性梅毒患者中IL-2与IL-10呈负相关(r=-0.760,P =0.000),与TNF-α呈正相关(r=0.633,P=0.000),而IL-10与INF-α无明显相关性(r=-0.063,P =0.575).结论 IL-2、IL-10和TNF-α均参与了梅毒的发病,IL-10表达上调可能是二期梅毒发生辅助性T(Th)1/Th2细胞免疫应答失衡的主要原因之一.%Objective To investigate the role of interleukin-2 (IL-2), interleukin-10( IL-10) and tumor necrosis factor-alpha (TNF-α) in the pathogenesis of early symptomatic syphilis. Methods Double-antibody sandwich enzyme-linked immunosorbent assay(DAbS-ELISA) was used to detect the serum levels of IL-2,IL-10 and TNF-α in 53 patients with early symptomatic syphilis (25 cases with primary syphilis and 28 cases with secondary syphilis) and 65 healthy subjects. Results Serum levels of IL-2, IL-10 and TNF-α in early symptomatic syphilis group were significantly higher than those in control group (P <0. 01). Serum levels of IL-2 and TNF-α in primary syphilis group were significantly higher than those in secondary syphilis group (P < 0. 01). Serum level of IL-10 in secondary syphilis group was significantly higher than that in primary syphilis group (P<0.01). IL-2 and IL-10 were negatively correlated in early symptomatic syphilis group (r = - 0. 760, P = 0. 000). IL-2 and TNF-α were positively correlated ( r = 0. 633, P = 0.000), and IL-10 and TNF-α were not correlated(r = -0.063,P =0.575). Conclusions IL-2, IL-10 and TNF-α are involved in the pathogenesis of syphilis. Up-expression of IL-10 may contribute to the helper T cell (Th) 1/Th2 immune response imbalance during the development of early symptomatic syphilis.