类孟买血型血清学鉴定及家系调查探讨

摘要

目的：探讨正确鉴定类孟买血型的方法，为罕见血型人群找寻更多相合供者。方法对4例正反定型不符者用ABO正反定型、Lewis血型鉴定、H抗原检测、红细胞吸收放散试验、冷抗体检测和检测唾液血型物质的中和抑制试验进行综合鉴定，并且进行家系调查。结果4例被检者均证实为类孟买血型。先证者c的妹妹也被证实为同型类孟买型。结论对正反定型不一致的标本要高度警惕，应加做其它必要的血清学试验。冷抗体检测还能为类孟买血型患者辅助选择合适的血液输注。家系调查不仅能查清类孟买血型的来源和遗传，而且可以提供更多相同稀有血型供者。%Objective To study the correct serology identification methods of para-Bombay phenotype and search compatible donors for rare blood type.Methods A total of 4 cases of positive and reverse typing discrepancy were identified comprehensively.ABO positive and reverse typing, Lewis blood type identification, H antigen detection, red blood cell absorption-elution test, cold antibody detection and hemagglutination inhibition test for blood type substance in saliva were performed.A family investigation was made.Results All the 4 cases were confirmed as para-Bombay phenotype.Proband c′s sister was also proved that she was the same para-Bombay phenotype.Conclusions The cases of positive and reverse typing discrepancy should be added other essential tests for para-Bombay phenotype.The detection of cold antibody assists in not only identifying para-Bombay phenotype but also selecting compatible blood for transfusion.Family investigation makes possible observe source and inheritance of para-Bombay phenotype and provide homogeneous donors for transfusion.