目的 构建胃癌转录调控网络,分析预后相关转录因子.方法 首先用基因芯片数据构建差异表达转录因子在癌组织中的共表达网络,进而发现共表达网络中的核心亚网络,然后用具有预后数据的合适表达谱芯片,根据构建的网络筛选预后相关的转录因子,采用样本量较大、有预后数据的表达谱芯片验证转录因子对胃癌患者预后是否有预测作用.结果 共发现4个核心网络、21个核心转录因子.结合转录因子核心网络,确定和预后相关的4个核心转录因子其中的3个和患者预后相关.结论 构建胃癌转录调控网络可为研究胃癌的潜在调控机制提供参考,并可能为将来胃癌的诊断治疗提供分子标志物及靶点.%Objective The purpose of this study is to construct the transcriptional regulatory network of gastric cancer ,and a-nalysis the prognostic transcription factors .Methods Firstly ,using gene chip data to construct co-expression networks of differen-tially expressed transcription factors in cancer tissues ,and then found the core subnetwork in the network ,and then screen the core transcription factors based on the microarray with prognosis data .We finally used a large amount of data to verify whether these transcription factors can predict the prognosis of gastric cancer patients .Results There are 21 core transcription factors of 4 core subnetworks .We identified 4 key transcription factors of that 3 transcription factors had obvious function of prediction . Conclusion We have constructed the transcriptional regulatory network which provides references for the potential regulatory mechanism of gastric cancer .They may provide molecular markers for future diagnosis of gastric cancer ,and possible targets for the treatments of gastric cancer .
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