生化标记方法在KM小鼠遗传结构分析中的应用

摘要

Objective To analyze the change of genetic structure of KM mice colonies between 1990 and 2010.Methods KM mice colonies (18 mice in 1990 and 50 mice in 2010) were monitored by biochemical markers under instruction of national standard GB/T 14927.1-2008.POPGENE 1.32 software was used to process the data.Results In 1990,average heterozygosity is 0.262 5,polymorphism information content is 0.206 5.In 2010,average heterozygosity is 0.161 5,polymorphism information content is 0.130 7.By compared with the two colonies,population differentiation coefficient is 0.122 4 and the genetic distance is 0.095 1.Conclusion After 20 years outdred,the KM colony has medium change of population differentiation,but still be considered as same strain from the point of population genetics.%目的 利用生化标记检测法分析1990年和2010年KM小鼠群体遗传结构的变化.方法 采用国家标准GB/T 14927.1-2008推荐的生化标记检测法对同一种群的来源KM小鼠(1990年的18只和2010年的50只)进行遗传质量检测,借助统计软件POPGENE l.32进行群体遗传结构分析.结果 1990年群体的平均杂合度是0.262 5,多态性信息含量是0.206 5.2010年群体的平均杂合度是0.161 5,多态性信息含量是0.130 7.群体间比较显示,群体间分化系数为0.1224,遗传距离为0.095 1.结论 经过20年的封闭繁育,该KM小鼠群体出现了中度的遗传分化,但仍属于同一品系(同种同属)的小鼠群体.