BAPTA-AM脂质体抗D-GalN/LPS所致的小鼠急性肝衰竭

摘要

To study the protective effects of BAPTA-AM liposome (BA-L) on D-galactosamine and lipopolysaccharide induced acute liver failure in mice,mice were treated with D-GalN and LPS to induce acute liver failure.BA-L of different doses was administered intravenously before D-GalN/LPS injection.The mortality,liver index,percent of water in liver,serum levels of alanine aminotransferase (ALT) and nitric oxide (NO),hepatic tissue glutathione (GSH),glutathione peroxidase (GSH-Px),superoxide dismutase (SOD) and malondialdehyde (MDA)were measured.Hematoxylin and eosin (HE) stain and Terminal deoxynucleotidyl transferasemediated UDP nick-end labeling (TUNEL) assay was used to evaluate pathologic changes and apoptotic index of hepatic tissue.BA-L could significantly decrease the mortality rate,the liver index,the serum levels of ALT and NO,the hepatic tissue level of MDA,increase the hepatic tissue GSH level,GSH-Px and SOD activity in ALF mice.BA-L alleviated D-GalN/LPS induced severe injury of hepatic tissue,and decreased apoptotic index remarkably.Besides,both of the potency and efficacy of BA-L are far higher than those of diammonium glycyrrhizinate.BA-L exert admirable protection against D-galactosamine and lipopolysaccharide induced acute liver failure in mice.%观察BAPTA-AM脂质体(BA-L)对D-GalN/LPS诱导的小鼠急性肝功能衰竭(ALF)保护作用并探讨其保肝作用机制.建立D-GalN/LPS致小鼠ALF模型,测定给予BA-L小鼠的24h死亡率,肝指数,肝含水质量分数,血清ALT和NO水平,肝匀浆GSH,GSH-Px,SOD,MDA水平,HE染色观察组织病理学改变,TUNEL法观察肝细胞凋亡并统计凋亡指数.结果表明:BA-L可显著减少小鼠死亡率,降低肝指数,血清ALT和NO值降低,可使肝匀浆GSH,GSH-Px,SOD升高,而MDA减少,HE染色可见肝组织病理情况明显改善,TUNEL染色可见肝细胞凋亡指数明显下降,其效果远优于甘草酸二铵,BA-L有上佳的抗ALF作用.