百令疏肝胶囊治疗免疫损伤性肝纤维化的实验研究

摘要

To explore the effect and mechanism of Corbrin shugan capsule on immunological hepatic fibrosis, the rat model of immunological hepatic fibrosis was established by intraperitoneal injection of pigs serum repeatedly. We determined the content of [II PC, LN, TIMP-1 in serum by ELISA, the content of ALB in serum by chemical color-imetric and the content of Hyp in liver tissue by HPLC. We observed Collagen deposition in liver tissue by masson staining, and measured the fibrosis area(S) by Motic Med 6. 0 motic digital medical image analysis system. Compared with the control group, the content of TIMP-1, LN in serum and Hyp in liver tissue evidently decreased with Corbrin shugan capsule groups for giving 270 and 450 mg · kg-1 dose. The hepatic fibrosis area evidently decreased with Corbrin shugan capsule groups for giving90, 270 and 450 mg· kg-l dose. The content of El PC and ALB in serum respectively evidently decreased and increased with Corbrin shugan capsule groups for giving 450 mg · kg-1 dose. Corbrin shugan capsule had significant therapeutic effect on immunological hepatic fibrosis induced by pig's serum. It shows Corbrin shugan capsule has significant positive regulatory effect on matrix metalloproteinase (MMP) > has passive regulatory effect on synthesis and deposition of extracellular matrix(ECM), can promote cell regeneration after injury, and can decrease the content of III PC and hepatic fibrosis area, so that it can slow down the process of liver fibrosis and recover liver function.%探讨百令疏肝胶囊对免疫损伤性肝纤维化的治疗作用及可能的作用机制.采用反复腹腔注射猪血清建立免疫损伤性肝纤维化模型,通过酶联免疫法测定血清Ⅲ型前胶原肽(ⅢPC)、层黏连蛋白(LN)、基质金属蛋白酶抑制因子-1 (TIMP-1)的质量浓度,化学比色法测定血清白蛋白(ALB)和肝组织羟脯氨酸(Hyp)的质量浓度,肝组织病理切片行Masson三色染色,观察胶原沉积情况,使用Motic Med 6.0数码医学图像分析系统测量肝纤维化面积(S).结果显示百令疏肝胶囊270、450 mg·kg-1剂量能明显降低猪血清致免疫损伤性肝纤维化大鼠血清中TIMP-1、LN水平和肝组织中Hyp水平,90、270、450 mg·kg-1剂量能明显降低猪血清致免疫损伤性肝纤维化大鼠肝纤维化面积(S),450 mg· kg-1剂量能明显降低猪血清致免疫损伤性肝纤维化大鼠血清中Ⅲ型前胶原肽水平,提高猪血清致肝纤维化大鼠血清中ALB水平.得到结论:百令疏肝胶囊对猪血清致免疫损伤性肝纤维化有较好的治疗作用.提示百令疏肝胶囊具有促进免疫损伤性肝纤维化大鼠基质金属蛋白酶活性而对细胞外基质的合成和沉积起到抑制作用,以及促进细胞损伤后再生、降低Ⅲ型前胶原肽质量浓度及肝纤维化面积的作用,从而减缓肝纤维化进程,恢复肝脏功能.