清热养阴方对多囊卵巢综合征（PCOS）模型大鼠卵巢激素及局部胰岛素抵抗的影响

摘要

Objective]To observe and understand the effect and mechanism of QingReYangYin Recipe(QRYYR) on the ovary hormone level and mRNA expressions of PI3K and PKB in adipose tissue around womb and ovary in rat model of polycystic ovary syndrome(PCOS).[Methods]Rat PCOS model was established by Poretsky method and divided randomly into four groups: model group, western medicine group,low dose QRYYR group and high dose QRYYR group. Normal rats were used as normal group. All rats were orally administrated for 14 days and were weighed every day.After 14 days treatment, serum sample of each rat was obtained for analysis of estradiol(E2),progesterone(P), testosterone(T) contents. Each ovary was collected and weighed for measurement of the ovarian index. The fat pad around womb and ovary was obtained for analyzing the mRNA expressions of PI3K and PKB by PCR. [Results]Compared with the model group, the serum E2 and P levels in QRYYR group were significantly higher but T level was significantly lower(P<0.05) than the model group. The levels of E2, P and T in high dose QRYYR group had no difference from that in the western medicine group and the normal group(P>0.05).The ovarian index in the QRYYR groups was significantly decreased as compared with the model group(P<0.05),and that in the high dose QRYYR groups had no difference with the normal group(P>0.05).The mRNA expressions of PI3K and PKB were significantly increased in the QRYYR groups as compared with the model group(P<0.05),and the expressions in high dose QRYYR group, western medicine group and the normal group had no significant difference from each other(P>0.05). [Conclusion] QRYYR could significantly increase the content of E2 and P in the rat serum, significantly decrease the content of T and the ovarian index. Meanwhile, it could significantly increase the mRNA expressions of PI3K and PKB in adipose tissue. It could improve the ovarian function and decrease the local insulin resistance. The effect of the high dosage group on PCOS was superior or similar to Metformin.%[目的]观察清热养阴方对多囊卵巢综合征（polycystic ovary syndrome，PCOS）模型大鼠卵巢激素及子宫卵巢旁脂肪组织中PI3KmRNA、PKBm－RNA的影响，了解其作用机制。[方法]按Poretsky法行PCOS大鼠造模,随机分为模型组、西药组、中药低剂量组、中药高剂量组，并设正常组。模型组和正常组用蒸馏水灌胃，高、低剂量组分别以中药39g·kg－1、13g·kg－1浓度灌胃，西药组用二甲双胍0．45g·kg－1浓度灌胃，各组分别灌胃14d，每日称重。处死取血，测定并比较各组大鼠雌二醇（E2）、孕酮（P）、睾酮（T）含量；取卵巢称重，计算卵巢指数。取子宫卵巢旁脂肪垫测定脂肪组织中PI3KmRNA、PKBmRNA表达量。[结果]给药后各治疗组大鼠血清E2、P含量均显著高于模型组(P<0．05)、T含量显著低于模型组(P<0．05)；高剂量组E2、P、T含量与西药组、正常组之间差异无统计学意义(P＞0．05)；各治疗组大鼠卵巢指数显著降低，与模型组相比差异有统计学意义（P<0．05），高剂量组与正常组差异无统计学意义(P＞0．05)；各治疗组均能显著增强脂肪组织中PI3KmRNA、PKBmRNA的表达，与模型组相比差异有统计学意义（P<0．05），高剂量组PI3KmRNA、PKBmRNA量与西药组、正常组之间差异无统计学意义(P＞0．05)。[结论]清热养阴方能提高大鼠E2、P，降低T；降低卵巢指数；增加PCOS模型大鼠子宫卵巢旁脂肪组织中PI3KmRNA、PKBmRNA的表达；从而改善大鼠胰岛素抵抗以及卵巢内分泌环境而恢复排卵。推测清热养阴方与二甲双胍可能具有相似的作用途径。