维药恰麻古总黄酮对 D-半乳糖致衰老小鼠氧自由基代谢的影响

摘要

Objective To investigate the effect of total flavonoids extract ofBrassica rapa L.on metabolism of oxygen free radicals in D-Galactose-induced aging mice.Methods The mice were randomly divided into 5 groups:normal group,model group,vitamin E group,high total flavonoids Magu and low dosage group.Except the normal group,the other 4 groups were daily morning neck back subcutaneous injection of D-galactose 150 mg/kg,model at the same time,high,low dose group were intragastrically just total flavonoids Magu 90,30 mg/kg,normal saline group and model group were administrated by volume.Sub-acute aging model in mice was established by D-galactose,while continuous irrigation taking just Brassica rapa L.flavonoids in 55 days and activities of SOD and GSH-PX,contents of MDA in the serum,brain, and liver of senile mice were detected.Results Total flavonoids extract of Brassica rapa L.markedly in-creased the activities of SOD and GSH-PX,significantly decreased contents of MDA in the serum,brain, and liver of aging mice.Conclusion Total flavonoids extract of Brassica rapa L.might have anti-aging effect,which is implemented by eliminating oxygen free radicals and rising activities of antioxidases.%目的：探讨维药恰麻古总黄酮对 D-半乳糖致衰老模型小鼠氧自由基代谢的影响及其抗衰老作用。方法随机将小鼠分成5组：正常组、模型组、维生素 E 组及恰麻古总黄酮高、低剂量给药组，除正常组外，其余4组每日上午均颈背部皮下注射 D-半乳糖150 mg/kg，造模同时，高、低剂量给药组分别灌服恰麻古总黄酮90、30 mg/kg，正常组和模型组灌服等容积的生理盐水，采用建立的 D-半乳糖致亚急性衰老小鼠模型，同时连续灌服恰麻古黄酮55 d。测定小鼠血清、肝脏和脑组织中超氧化物歧化酶（SOD）、谷胱甘肽还原酶（GSH-PX）活性、丙二醛（MDA）的含量。结果恰麻古总黄酮可显著提高 D-半乳糖所致衰老小鼠血清、脑和肝脏组织中 SOD、GSH-PX 活力，降低 MDA 含量，与衰老模型组相比差异有统计学意义（P ＜0．05）。结论恰麻古总黄酮可通过增强机体清除氧自由基能力，提高抗氧化酶活性而发挥抗衰老作用。