东北鹤虱提取物LEGPS-Ⅱ对小鼠巨噬细胞氧化损伤的保护作用

摘要

Objective: To research the protective effects of Lappula Echinata Gilib extract (LEGPS - Ⅱ ) on murine peritoneal macrophage with oxidative damage. Methods: The peritoneal macrophages from KM mice were cultivated in this experiment. The macrophage were divided into 5 groups randomly: Control group; H2O2 group; 30, 60, 120 μmol/L LEGPS- Ⅱ groups. The viabilities of macrophage were detected using MTT method. Activity of total superoxide dismutase(T-SOD), inducible nitric oxide synthase(iNOS)and content of malondialdehyde (MDA) and nitric oxide(NO) in the culture medium were detected using colorimetric method. Results: Compared with control group, in the group treated with H2O2, the cell viabilities was decreased significantly (P<0.01), the content of NO and the activity of iNOS were increased significantly (P<0.01), the activity of T-SOD was decreased (P<0.01), the content of MDA were increased significantly (P<0.01); Compared with H2O2 group, in LEGPS-Ⅱ groups, the cell viability was increased significantly (P<0.05, P<0.01), the content of NO and the activity of iNOS were decreased significantly (P<0.05, P<0.01), the activity of T-SOD was increased (P<0.05, P<0.01), and the content of MDA were decreased significantly (P<0.01), with the concentration dependent. Conclusion: LEGPS-Ⅱ can protect peritoneal macrophage with H2O2 damage in different stages through its antioxidant effects.%目的:研究东北鹤虱提取物LEGPS-Ⅱ对小鼠巨噬细胞氧化损伤的保护作用.方法:采用昆明种小鼠,培养腹腔巨噬细胞(MΦ),实验分为5组:正常对照组;H2O2氧化损伤组;3个浓度的LEGPS-Ⅱ(30、60、120μmol/L)保护组.用MTT法测定细胞存活率,比色法测定细胞培养液中一氧化氮(NO)含量、诱导型一氧化氮合酶(iNOS)活力、总超氧化物歧化酶(T-SOD)活力、丙二醛(MDA)含量.结果:与正常对照组相比,经H2O2处理过的氧化损伤组,细胞存活率明显降低(P＜0.01),NO的含量、iNOS的活力明显升高(P＜0.01),T-SOD活力明显下降(P＜0.01),MDA含量明显升高(P＜0.01);与H2O2损伤组相比,给LEGPS-Ⅱ各组均显著提高细胞存活率(P＜0.05,P＜0.01);使细胞培养液中NO的含量、iNOS的活力显著降低(P＜0.05,P＜0.01),T-SOD活力显著提高(P＜0.05,P＜0.01),MDA含量显著降低(P＜0.01),且呈现剂量依赖性.结论:东北鹤虱提取物LEGPS-Ⅱ可通过其抗氧化作用而在不同阶段保护经H2O2损伤的巨噬细胞